玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁  >  技術(shù)文章  >  4T1 小鼠乳腺癌細(xì)胞

4T1 小鼠乳腺癌細(xì)胞

更新時間:2016-06-22      點(diǎn)擊次數(shù):2486

細(xì)胞名稱:4T1 小鼠乳腺癌細(xì)胞
介紹:4T1 是從410.4瘤株中未經(jīng)誘變篩得的6-硫鳥嘌噙抗性細(xì)胞株。 當(dāng)注射到BALB/c 小鼠中時,4T1自發(fā)產(chǎn)生高轉(zhuǎn)移腫瘤,可轉(zhuǎn)移到肺,肝,淋巴結(jié)和大腦,同時在注射部位形成始發(fā)灶。 誘導(dǎo)轉(zhuǎn)移時不需要摘除始發(fā)灶。 4T1細(xì)胞在BALB/c小鼠中的生長與轉(zhuǎn)移特性與人體中的乳腺癌十分相近。這種腫瘤是人VI期乳腺癌的動物模型。 4T1-誘導(dǎo)的腫瘤在手術(shù)后及未手術(shù)情況下轉(zhuǎn)移的動力學(xué)相近,可以用作手術(shù)后及未手術(shù)模型。 跟其他腫瘤模型相比,由于4T1的抗6-硫鳥嘌噙特性,微小的轉(zhuǎn)移細(xì)胞團(tuán)(少到僅僅1個)也可以在許多遠(yuǎn)端器官中檢測到。 沒必要數(shù)淋巴結(jié)或稱重器官
形態(tài)特性       上皮細(xì)胞
生長特性      貼壁生長
傳代方法       消化3-5分鐘。1:2。3天內(nèi)可長滿。
支原體檢測      陰性
下面是ATCC的介紹

4T1 (ATCC® CRL-2539™)
Organism    Mus musculus, mouse
Tissue     mammary gland
Product Format     frozen4T1 小鼠乳腺癌細(xì)胞
Morphology     epithelial
Culture Properties     adherent
Biosafety Level     1
Disease     This tumor is an animal stage IV human breast cancer.
Strain     BALB/cfC3H
Applications    
The tumor growth and metastatic spread of 4T1 cells in BALB/c mice very closely mimic human breast cancer. This tumor is an animal model for stage IV human breast cancer.

4T1-induced tumors can be used as a post-operative model as well as a non-surgical model because the 4T1-induced tumor metastasizes spontaneously in both models with similar kinetics.
Storage Conditions     liquid nitrogen vapor temperature
Derivation    
4T1 is a 6-thioguanine resistant cell line selected from the 410.4 tumor without mutagen treatment.
Tumorigenic     Yes
Effects    4T1 小鼠乳腺癌細(xì)胞
Yes, forms tumors and metastasizes in BALB/c mice
Comments    
When injected into BALB/c mice, 4T1 spontaneously produces highly metastatic tumors that can metastasize to the lung, liver, lymph nodes and brain while the primary tumor is growing in situ.
The primary tumor does not have to be removed to induce metastatic growth.
Because 4T1 is resistant to 6-thioquanine, micro-metastatic cells (as few as 1) can be detected in many distant site organs with better accuracy that most tumor models. There is no need to count nodules or weight target organs.

Complete Growth Medium     The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Phenol Red is a structural mimic for estrogen. Therefore the estrogenic activity of phenol red should be considered in any studies that utilize estrogen-responsive cells in culture (Ref). The Phenol Red-free version of RPMI 1640, is ATCC catalog No. 30-2602.

Subculturing    
NOTE: the cells should not be allowed to become confluent, subculture at 80% of confluence. Remove medium, and rinse with 0.25% trypsin-0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation    
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions    
Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
野外真人实战CS| 欧美熟妇另类久久久久久多毛| 日韩在线视频| 亚洲精品成人网站在线观看| 亚洲欧美婷婷五月色综合| 亚洲日韩乱码久久久久久| 日本乱偷互换人妻中文字幕| 精品熟女少妇AV免费久久| 小SAO货CAO得你舒服吗男男| 年轻护士的滋味中文字幕| 日本16岁RAPPER| 亚洲国产精品无码久久一区二区 | 久久久精品国产SM最大网站| 精品久久久久成人码免费动漫| 中国极品少妇XXXXX| 亚洲AV天堂一区二区香蕉| 暴虐SM灌浣肠调教A片男男| 亚洲熟女乱综合一区二区| 亚洲码欧美码一区二区三区| 不断作死后我成了白月光| 亚洲精品久久久口爆吞精| 艳妇臀荡乳欲伦交换| 精品国产污污免费网站AⅤ| 吴北唐紫怡小说免费阅读| 成视频年人黄网站免费视频| 很黄的吸乳A片三男一女| 久久久久亚洲AV片无码下载蜜桃| 极大满足求知欲的视频| 推特怎么在国内使用| 女上男下啪啪激烈高潮无遮盖| 国产精品18久久久久久VR| 变态捡到女婴H养成调教| 98国产精品综合一区二区三区| 人妻少妇精品专区性色AV| 亚洲av噜噜一区二区三区| 国精品人妻无码一区二区三区软件 | 无码人妻精品丰满熟妇区| 最近中文字幕在线资源| 最近中文字幕在线MV视频在线| 被群CAO的合不拢腿H纯肉视频| 免费看美女裸露双乳洗澡视频|