玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁  >  技術(shù)文章  >  TOV-21G 人卵巢癌細(xì)胞

TOV-21G 人卵巢癌細(xì)胞

更新時(shí)間:2016-08-01      點(diǎn)擊次數(shù):2385
Designations:TOV-21G
Depositors: University of Montreal
Biosafety Level:1
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:adherent
Organism:Homo sapiens deposited as human
Morphology:epithelial
 
Source:Organ: ovary 
Tumor Stage: grade 3, stage III 
Disease: primary malignant adenocarcinoma; clear cell carcinoma
Cellular Products:keratin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24.
Tumorigenic:Yes
Oncogene:p53 + (wild type)
DNA Profile (STR):Amelogenin: X 
CSF1PO: 13,15 
D13S317: 11,12 
D16S539: 10,12 
D5S818: 12,13 
D7S820: 12 
THO1: 7,9.3 
TPOX: 8,11 
vWA: 17
Cytogenetic Analysis:47, XX, +10 [49408 ]
Age:62 years
Gender:female
Comments:This cell line was initiated in October of 1991 from a patient of French-Canadian descent with no family history of ovarian cancer. 
Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24.
Propagation:ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
    Temperature: 37.0°C
Subculturing:Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.05% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37?C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended 
Medium Renewal: Every 3 to 4 days
Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
Storage temperature: liquid nitrogen vapor phase
Related Products:recommended serum:ATCC 30-2020
References:

42090: Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998
49408: Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

CoC1人卵巢癌細(xì)胞系Lec1倉鼠卵巢細(xì)胞
A2780人卵巢癌細(xì)胞OVAC卵巢癌細(xì)胞
Anglne人卵巢癌細(xì)胞系NIH:OVCAR-3人卵巢癌細(xì)胞
Caov-3人乳突狀卵巢腺癌細(xì)胞PA-1人卵巢畸胎瘤細(xì)胞
CHO中國倉鼠卵巢細(xì)胞Pr-HNV8菜青蟲卵巢細(xì)胞
CHO/dhFr-倉鼠卵巢細(xì)胞,二氫葉酸還原酶缺陷RP70-a-CHO-0.8MTX中國倉鼠卵巢細(xì)胞
CHO-K1中國倉鼠卵巢細(xì)胞SF9晚秋粒蟲卵巢細(xì)胞
CHO-pt轉(zhuǎn)血小板基因倉鼠卵巢細(xì)胞SKOV3人卵巢癌細(xì)胞
CHO-S中國倉鼠卵巢懸浮細(xì)胞SKOV-3/DDP卵巢癌順鉑耐藥株
CoC1人卵巢癌細(xì)胞系SW1910人卵巢癌細(xì)胞
CoC2 卵巢癌 SW626人卵巢腺癌細(xì)胞
CTLA4 Ig-24中國倉鼠卵巢細(xì)胞TOV-21G人卵巢癌
ES-2人卵巢透明細(xì)胞癌ZA1轉(zhuǎn)S9基因倉鼠卵巢細(xì)胞
HO-8910人卵巢癌細(xì)胞ZA6轉(zhuǎn)S9基因倉鼠卵巢細(xì)胞
HO-8910PM人高轉(zhuǎn)移卵巢癌細(xì)胞 Cho-K1倉鼠卵巢細(xì)胞亞株
HOC1卵巢癌細(xì)胞SK-OV-3人卵巢癌細(xì)胞
Lec1倉鼠卵巢細(xì)胞Sf9昆蟲卵巢細(xì)胞
CHO-K1倉鼠卵巢細(xì)胞亞株ES-2人卵巢透明細(xì)胞癌
CHO/dhFr-倉鼠卵巢細(xì)胞,二氫葉酸還原酶缺陷SK-OV-3人卵巢癌細(xì)胞TOV-21G 人卵巢癌細(xì)胞TOV-21G 人卵巢
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
女女互磨互喷水高潮LES呻吟| 欧美AV蜜桃一区二区蜜桃| 99蜜桃在线观看免费视频网站| 国产av精品国语对白国产| 久久久精品日本一区二区三区| 2022最新韩国理伦片在线观看| 被两个男人按住吃奶好爽| 日本中文字幕| 久久午夜夜伦鲁鲁片无码免费| 精品丝袜av在线观看视频| 破了亲妺妺的处免费视频国产| 日韩欧美一区二区三区免费观看| 久久亚洲精品AB无码播放| 日本亚洲色大成网站WWW久久| 天美传媒剧国产剧情MV公司| 国产麻豆一区二区三区精品视频 | 色情久久久av熟女人妻网站| 亚洲精品国产精品| 麻豆国产av国片精品有毛| 国产JAZZ亚洲护士无码| 18无码粉嫩小泬无套在线观看| 10000拍拍18勿入免费看| 老少做爰XXXXHD老少配| 妓女嫖客叫床粗话对白| 久久久精品人妻一区二区三区 | 国产伦理一区二区| av免费网站在线观看| 久久国产一区二区三区| 成人做受视频试看120秒| 免费视频完整版软件下载| 性欧美丰满熟妇XXXX性| 帅的中国大陆GARY1609| 97久久久久人妻精品区一| 最近日本韩国高清免费观看| 国产V亚洲V天堂无码| 成人免费电影网站| 体育生翘臀公0被猛攻GΑY| 精品无码国产一区二区三区51安| 中文无码亚洲色偷偷A片| 成A人片亚洲日本久久| 精品欧美一区二区三区久久久|