玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1739AGS 人胃腺癌細(xì)胞

AGS 人胃腺癌細(xì)胞

簡(jiǎn)要描述:CRL-1739 AGS 人胃腺癌細(xì)胞
上海復(fù)祥生物公司現(xiàn)貨供應(yīng)美國(guó)典型培養(yǎng)物保藏中心(ATCC)各類細(xì)胞,*。 手機(jī) xiangfbio.歡迎各位老師來電咨!

  • 產(chǎn)品型號(hào):CRL-1739
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:2260

產(chǎn)品分類

Product Category

詳細(xì)介紹

RL-1739 AGS 人胃腺癌細(xì)胞 的詳細(xì)介紹

AGS 人胃腺癌細(xì)胞

ATCC® Number:  CRL-1739™

Designations:  AGS

Depositors:   SC Barranco

Biosafety Level: 2

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial


Source: Organ: stomach

Disease: gastric adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Isolation:  Isolation date: 1979

Applications: transfection host (Nucleofection technology from Lonza)

Tumorigenic: Yes

Cytogenetic Analysis: This is a hyperdiploid human cell line. The modal chromosome number was 49, occurring in 60% of cells. The rate of polyploidy was 3.6%. Single copy each for der(8)t(1;8) (q12;p23), der(19)t(19;?) (q13.6;?), minute chromosome M3, and C-group-like M12 was seen in all cells. The origins of both M3 and M12 defied identification presently. The t(13q14q) occurred in some. Generally there were three copies for N20, and single copy for X, N8 and N18. Occasionally there were three copies for N14.

Age:  54 years

Gender:  female


Ethnicity:  Caucasian

Comments: The AGS cell line was derived from fragments of a tumor resected from a patient who had received no prior therapy. The cells have a plating efficiency of 34% in the medium below. The line was cured at the ATCC of a prior mycoplasma infection .Subsequently, AGS has been determined to be infected with Parainfluenza type 5 (PIV5 formerly known as SV5). [PubMed: 17509637]

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time:  20 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

References: 22748: Barranco





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
亚洲精品一区久久久久久| 国产精品久久久久精品三级卜| 最近中文字幕在线中文一页| 欧美精品99久久久啪啪| 看AV免费毛片手机播放| 精品国产污污免费网站AⅤ| 最近国语视频在线观看免费播放| 国产成人一区二区三区影院 | 国产乱妇无码大黄aa片| 黑人粗大无码AV人妻一区| 蜜桃色欲av久久无码精品| 亚洲av天堂综合网久久| 少妇高h肉辣全集目录| 日韩精品在线观看| 最近2019中文字幕电影免费看| 国产大片b站免费观看推荐| 亚洲AV永久无码精品一福利| 国产精品自产拍高潮在线观看| 天码AV无码一区二区三区四区| 日本真人做爰免费的视频| 丰满白嫩少妇肉肉大HD| 老人玩小处雌女视频| 色欲久久综合亚洲精品蜜桃| 99热最新地址| 小肚子鼓起按H宫交| 日本边添边摸边做边爱60分钟| 亚洲精品一区中文字幕乱码| 99RE热这里只有精品视频| 粗大挺进尤物人妻中文字幕| 久久久久亚洲av片无码v| 久久人妻少妇嫩草AV蜜桃 | 国产免费一区二区在线a片视频| 久久久久亚洲精品中文字幕| 精国产品一区二区三区A片| 国产+日韩+另类| 欧美日韩精品一区二区在线播放| 99久久精品费精品蜜臀av| 中文字幕AV无码免费一区| 精品丝袜人妻久久久久久| 天天夜夜草草久久伊人69堂| 最近日本MV字幕免费观看|