玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-1739AGS 人胃腺癌細(xì)胞

AGS 人胃腺癌細(xì)胞

簡(jiǎn)要描述:CRL-1739 AGS 人胃腺癌細(xì)胞
上海復(fù)祥生物公司現(xiàn)貨供應(yīng)美國(guó)典型培養(yǎng)物保藏中心(ATCC)各類細(xì)胞,*。 手機(jī) xiangfbio.歡迎各位老師來電咨!

  • 產(chǎn)品型號(hào):CRL-1739
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:2260

產(chǎn)品分類

Product Category

詳細(xì)介紹

RL-1739 AGS 人胃腺癌細(xì)胞 的詳細(xì)介紹

AGS 人胃腺癌細(xì)胞

ATCC® Number:  CRL-1739™

Designations:  AGS

Depositors:   SC Barranco

Biosafety Level: 2

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial


Source: Organ: stomach

Disease: gastric adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Isolation:  Isolation date: 1979

Applications: transfection host (Nucleofection technology from Lonza)

Tumorigenic: Yes

Cytogenetic Analysis: This is a hyperdiploid human cell line. The modal chromosome number was 49, occurring in 60% of cells. The rate of polyploidy was 3.6%. Single copy each for der(8)t(1;8) (q12;p23), der(19)t(19;?) (q13.6;?), minute chromosome M3, and C-group-like M12 was seen in all cells. The origins of both M3 and M12 defied identification presently. The t(13q14q) occurred in some. Generally there were three copies for N20, and single copy for X, N8 and N18. Occasionally there were three copies for N14.

Age:  54 years

Gender:  female


Ethnicity:  Caucasian

Comments: The AGS cell line was derived from fragments of a tumor resected from a patient who had received no prior therapy. The cells have a plating efficiency of 34% in the medium below. The line was cured at the ATCC of a prior mycoplasma infection .Subsequently, AGS has been determined to be infected with Parainfluenza type 5 (PIV5 formerly known as SV5). [PubMed: 17509637]

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time:  20 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

References: 22748: Barranco





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
51国偷自产一区二区三区| 最近中文字幕在线看免费完整版| 色喜国模私密浓毛私拍人体图片| 调教室捆绑白丝JK震动捧娇喘| 欧美与黑人午夜性猛交久久久| 久久久久噜噜噜亚洲熟女综合| 欧美成人看片一区二区三区尤物| 对白脏话肉麻粗话AV| 精品一区二区三区| H女主从小被C到大1V1| 蜜桃AV色偷偷AV老熟女| 亚洲2022国产成人精品无码区 | 亚洲av无码一区二区三区在线| 性一交一乱一乱一视一频| 色欧美片视频在线观看| 亚洲成A人片在线观看无码专区| 国产日韩欧美一区二区三区| 和子发生了性关系的免费视频| 暴力调教一区二区三区| 国产亚洲精品久久久久秋霞| 久久99国产精品久久99| 国产VIVODESHD精品| 老头趴在两腿中间添我| 男人的天堂AV| 波多野结衣在线视频| 99E久热只有精品8在线直播| 风韵多水的老熟妇| 国产日韩精品中文字无码| 天天摸夜夜摸摸到高潮| 风流老熟女一区二区三区| 男人用嘴巴添女人的坏处| 国产A级特黄的片子| 蒙古少妇BBB多毛露屁| 无码精品人妻一区二区三区漫画 | 日本无码精油按摩WWW视频| 波多也结衣无码精品AV在线观看 | 真人做人60分钟啪啪免费看| 娇喘抽搐高潮喷水A片免费观看 | 少妇高潮惨叫久久久久久| 欧美大片免费播放器| 亚洲精品又粗又大又爽a片 |