玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠正常細(xì)胞>PCEMM01美國(guó)Sox1-GFP報(bào)告基因小鼠胚胎干細(xì)胞

美國(guó)Sox1-GFP報(bào)告基因小鼠胚胎干細(xì)胞

簡(jiǎn)要描述:美國(guó)Sox1-GFP報(bào)告基因小鼠胚胎干細(xì)胞
上海復(fù)祥生物公司現(xiàn)貨供應(yīng)美國(guó)典型培養(yǎng)物保藏中心(ATCC)各類細(xì)胞菌種,*。 手機(jī) xiangfbio.歡迎各位老師!

  • 產(chǎn)品型號(hào):PCEMM01
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:2488

產(chǎn)品分類

Product Category

詳細(xì)介紹

美國(guó)Sox1-GFP報(bào)告基因小鼠胚胎干細(xì)胞的介紹

美國(guó)Sox1-GFP報(bào)告基因小鼠胚胎干細(xì)胞是美國(guó)PrimCells干細(xì)胞:PrimCells提供多種報(bào)告基因敲進(jìn)的小鼠胚胎干細(xì)胞,該類細(xì)胞皆在原始基因座上敲入熒光報(bào)告基因,從而使熒光蛋白的表達(dá)能真實(shí)反映原基因座附近的表達(dá)調(diào)控狀況。該類細(xì)胞作為研究胚胎干細(xì)胞自更新和定向分化的有力工具,受到多家著名研究所研究人員的青睞。


Species:Mus MusculusCatalog Number:PCEMM01
Cell Number:~2x106Storage/Shipping:Dry Ice


Introduction:


Sox1 is a transcriptional factor from SOX protein family. It is one of the earliest and most specific markers for neural progenitors and expressed throughout the central neural system during early embryo development1, 2. PrimCells is proud to isolate and provide to the research community the culture of Sox1-EGFP ESCs from transgenic knock-in mice. These cells were cultured in standard ESCs medium on gelatin coated plates and be used as a powerful tool to examine the process by which mESCs acquire neural identity.


ES cell culture and monolayer neural differentiation procudures

Sox1-GFP mESCs should be maintained in the stem cell culture medium to keep the pluripotent state (Figure 1).

For monoculture neural differentiation, dissociate and plate the undifferentiated Sox1-GFP mESCs  on to 0.1% gelatin-coated tissue culture plastic at a density of 1-3x104/cm2 in serum-free N2B27 medium. Renew N2B27 medium every 2 days. GFP positive cells can be observed on Day 5 (Figure 2).

美國(guó)Sox1-GFP報(bào)告基因小鼠胚胎干細(xì)胞


Figure 1 | Sox1-GFP mESCs express typical ES cell markers


美國(guó)Sox1-GFP報(bào)告基因小鼠胚胎干細(xì)胞

Figure 2 |  Monoculture neuronal differentiation of Sox1-GFP mESCs on Day 5

Growth Medium

Embryonic stem cell medium

DMEM:   415ml

FBS: 75ml (HYCLONE LABORATORIES INC, Cat# SH30070.03EH)

L-Glutamine: 5ml

MEM NEAA: 5ml

1-Thioglycerol: 4.3ul

LIF: 50ul LIF: 50ul (10ng/ml final concentration)  (PrimCells, Cat# PFHSA01)

Total Volume: 500ml

N2B27 medium for neural differentiation

N2B27 is a 1:1 mixture of DMEM/F12 (Gibco, Paisley, UK) supplemented with modified N2 (25 µg/ml insulin, 100 µg/ml apo-transferrin, 6 ng/ml progesterone, 16 µg/ml putrescine, 30 nM sodium selenite and 50 µg/ml bovine serum albumin fraction V (Gibco)) and Neurobasal medium supplemented with B27 (both from Gibco).

Alternatively, Ndiff227 (StemCells INC, Cat# SCS-SF-NB-02) is ready-to-use medium for the neural differentiation of mESCs  in adherent monoculture conditions as described in Ying QL et al (2003)2.

References

1.   Aubert, J. et al. Screening for mammalian neural genes via fluorescence-activated cell sorter purification of neural precursors from Sox1-gfp knock-in mice. Proc Natl Acad Sci U S A 100 Suppl 1, 11836-11841 (2003).

2.   Ying, Q.L., Stavridis, M., Griffiths, D., Li, M. & Smith, A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat Biotechnol 21, 183-186 (2003).

美國(guó)PrimCells品牌介紹

PrimCells是位于美國(guó)加州圣迭戈的一家專業(yè)致力于細(xì)胞產(chǎn)品研發(fā)和解決方案提供的業(yè)內(nèi)技術(shù)。

公司匯集了資深的生物醫(yī)藥技術(shù)專家,獨(dú)立開發(fā)了多項(xiàng)原代細(xì)胞和干細(xì)胞相關(guān)的專有技術(shù)。 公司以“細(xì)胞研究的優(yōu)選擇"為宗旨,自主研發(fā)超過(guò)70種創(chuàng)新產(chǎn)品,多個(gè)特種細(xì)胞市場(chǎng)的空白。例如其誘導(dǎo)性表達(dá)細(xì)胞庫(kù)和高效3D皮膚培養(yǎng)系統(tǒng),皆為該類產(chǎn)品目前市場(chǎng)上少數(shù)的規(guī)?;?yīng)商。此外,公司以高質(zhì)量的數(shù)據(jù)與快速穩(wěn)定的項(xiàng)目完成時(shí)間,長(zhǎng)期為多家制藥公司提供解決方案,獲得用戶廣泛贊譽(yù)。

PrimCells產(chǎn)品線:

  • 報(bào)告基因干細(xì)胞        2. 原代細(xì)胞(含報(bào)告基因原代細(xì)胞)               3. 誘導(dǎo)性穩(wěn)定細(xì)胞株

4. 人源重組蛋白               5.   創(chuàng)新型培養(yǎng)試劑

PrimCells 解決方案

1.誘導(dǎo)性穩(wěn)定細(xì)胞株開發(fā) 2. 原代細(xì)胞開發(fā) 3. 藥物體外毒理和效能測(cè)試 4. 3D-皮膚培養(yǎng)

PrimCells合作伙伴及客戶:

A.生物制藥公司:輝瑞(Pfizer), 諾華(Novartis), 禮來(lái)(Eli2  Lilly),. Amgen, EMD Millipore, Regeneron等公司的毒理和醫(yī)藥研發(fā)部門

B.科研機(jī)構(gòu):冷泉港實(shí)驗(yàn)室,哈佛醫(yī)學(xué)院, 麻省理工(MIT),, UCSF, NYU, Salk, UIUC, USC等
















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
白雪被出租车司机强好爽| 中文字幕一区二区三区乱码 | 小雪被老汉各种姿势玩| 日本不卡一区二区三区| 99蜜桃臀久久久欧美精品网站| 免费观看性生交大片| 少妇被又大又粗又爽毛片欧美| 少妇被粗大的猛烈进出免费视频| 人人爽人人爽人人片AV| 娇小搡BBBB搡BBBB| 天堂VA蜜桃一区二区三区| 成免费CRM直接看| 成人性生交大片免费看9| 欧美日韩国产| 成熟人妻换XXXX| 黑人巨大粗物挺进了少妇| 99久久亚洲精品无码毛片| 乱码丰满人妻一二三区麻豆| 边吃奶边被躁欧美三级| 玩弄japan白嫩少妇hd小说| 97在线观视频免费观看| 国产裸体舞一区二区三区| 偷窥美女洗澡一区二区三区| 无码人妻少妇久久中文字幕| 欧美人妻日韩精品| 无码欧亚熟妇人妻AV在线| 51久久成人国产精品麻豆| 国产午夜精品AV一区二区| 久久精品国产亚洲7777| 性色AV浪潮AV色欲AV| 中文无码AV一区二区三区| 少妇BBWBBW性生话| 亚洲精品久久久口爆吞精| 成人免费视频| 伊人久久大香线蕉综合75| 69国产精品成人无码视频| 色欲色香天天天综合网WWW| 国产我和子的乱视频| 蜜芽796.COO永不失联| 欧美性大战久久久久久久 | 娇喘抽搐高潮喷水A片免费观看 |