玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細胞庫>人正常細胞>CRL-2190HK-2 細胞,人腎近曲小管上皮細胞

HK-2 細胞,人腎近曲小管上皮細胞

簡要描述:HK-2 細胞,人腎近曲小管上皮細胞
原代細胞|細胞系|細胞株|菌種;細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2190
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-15
  • 訪  問  量:2040

產(chǎn)品分類

Product Category

詳細介紹

HK-2 細胞,人腎近曲小管上皮細胞

ATCC® Number:CRL-2190™    Price:$272.00
Designations:HK-2

Depositors:RA Zager

Biosafety Level:2 [Cells Contain Papilloma viral DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Organ: kidney, cortex
Tissue: proximal tubule
Cell Type: human papillomavirus 16 (HPV-16) transformed


Cellular Products:alkaline phosphatase; gamma glutamyltranspeptidase; leucine aminopeptidase; acid phosphatase; cytokeratin; alpha 3, beta 1 integrin; fibronectin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
HK-2 細胞,人腎近曲小管上皮細胞

Receptors:epidermal growth factor (EGF), expressed

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 13

D13S317: 9

D16S539: 11,12

D5S818: 12

D7S820: 10,11

THO1: 9

TPOX: 8,9

vWA: 17,18



Age:*****

Gender:male

Comments:HK-2 (human kidney 2) is a proximal tubular cell (PTC) line derived from normal kidney.

The cells were immortalized by transduction with human papilloma virus 16 (HPV-16) E6/E7 genes.

The recombinant retrovirus vector pLXSN 16 E6/E7 containing the HPV-16 E6/E7 genes was used to transfect the ectotropic packaging cell line Psi-2.

Virus produced by the Psi-2 cells was used to infect the amphotropic packaging cell line PA317 (see ATCC CRL-9078).

Virus produced by the PA317 cells was used to transduce primary PTCs.

Although pLXSN 16 E6/E7 also confers resistance to neomycin, selection in G418 was not used to isolate transduced clones.

The cell line appears to be derived from a single cell based on Southern and FISH analysis.

The E6/E7 genes are present in the HK-2 genome as determined by PCR.

The cells retain a phenotype indicative of well differentiated PTCs.

They are positive for alkaline phosphatase, gamma glutamyltranspeptidase, leucine aminopeptidase, acid phosphatase, cytokeratin, alpha 3,beta 1 integrin, and fibronectin.

The cells are negative for factor VIII related antigen, 6.19 antigen and CALLA endopeptidase.

HK-2 cells retain functional characteristics of proximal tubular epithelium such as Na+ dependent / phlorizin sensitive sugar transport and adenylate cyclase responsiveness to parathyroid, but not to antidiuretic hormone.

The cells are capable of gluconeogenesis as evidenced by their ability to make and store glycogen.

HK-2 cells are anchorage dependent.

The cells will not grow in methylcellulose, soft agar or suspension.

HK-2 cells can reproduce experimental results obtained with freshly isolated PTCs.



Propagation:ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
  • 0.05 mg/ml BPE - provided with the K-SFM kit

  • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Growth Conditions: Cell growth is dependent on epidermal growth factor. The cells should not be allowed to become confluent. Subculture at 80% of confluence.



Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.05% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产精品免费视频| 好爽毛片一区二区三区四无码三飞| 山村最原始的肉欲伦情| 汽车上开两个的花苞| 国产精品久久久久久麻豆一区| 妺妺窝人体色WWW在线观看| 欧美激情性视频高清在线观看| 女人下边紧了好还是松点好| 好爽…又高潮了毛片喷水| 秋霞无码av久久久精品小说| 丰满中国少妇和黑人玩| 国产SUV精品一区二区四| 好男人好资源影视在线| 国产AV日韩A∨亚洲AV电影| 双乳被和尚揉着玩弄在线看 | 麻豆AV天堂一区二区香蕉| 久久久久亚洲AV无码| 久久精品国产亚洲AV无码偷窥| 午夜福利在线观看| 一本一道久久A久久精品综合| AV色综合久久天堂AV色综合在| 中国妇被黑人XXX猛交| 国产国拍亚洲精品MV在线观看| 大肉蒂被嘬的好爽H公主| 欧美日韩国产| 一边吃奶一边哭乱抻又乱扭| 国产精品久久久久无码av色戒 | 欧美AV噜噜狠狠网址蜜桃| 久久精品卫校国产小美女 | 欧洲熟妇色xxxxx欧美老妇伦| 国产A级特黄的片子| 在厨房被C到高潮A毛片奶水| 国产亚洲精品久久久久久无码下载| 亚洲一区二区三区写真| AV中文字幕| 娇小萝被两个黑人用半米长| 99国产精品久久久久久久成人| 丰满少妇人妻久久久久久| xxxx丰满小少妇女自小说| 国产特级毛片AAAAAAA高清| 久久久精品一区二区三区免费 |