玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CCL-186IMR-90細(xì)胞, 人胚肺成纖維細(xì)胞

IMR-90細(xì)胞, 人胚肺成纖維細(xì)胞

簡(jiǎn)要描述:IMR-90細(xì)胞, 人胚肺成纖維細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CCL-186
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問  量:2110

產(chǎn)品分類

Product Category

詳細(xì)介紹

IMR-90細(xì)胞, 人胚肺成纖維細(xì)胞

ATCC® Number:  CCL-186™

Designations:  IMR-90

Depositors:   WW Nichols

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: fibroblast

IMR-90細(xì)胞, 人胚肺成纖維細(xì)胞

Source: Organ: lung

Disease: normal

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host (Nucleofection technology from Lonza)

Virus Susceptibility: Human poliovirus 1

Human poliovirus 2

Varicella-Zoster

Herpes simplex virus 1

Herpes simplex virus 2

Human poliovirus 3

Vaccinia virus

Human herpesvirus 5

Vesicular stomatitis virus

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,13

D13S317: 11,13

D16S539: 10,13

D5S818: 12,13

D7S820: 9,12

THO1: 8,9.3

TPOX: 8,9

vWA: 16,19

Cytogenetic Analysis: normal human female; diploid; stable

Isoenzymes:  G6PD, B

Age:  16 weeks gestation

Gender:  female

Ethnicity:  Caucasian

Comments: The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus. [22381]

The division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains.

The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence. [22381]

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended

Medium Renewal: Every 3 to 4 days

Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22381: Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science 196: 60-63, 1977. PubMed: 841339

32932: Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642

33041: Ostlund RE Jr., et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产69堂一区二区三区在线观看| 国产农村又大又粗A片| 777爽死你无码免费看一二区| 精品国产AV一区二区三区| 亚洲人成人无码网WWW国产| 久久久久无码精品国产| 岳把我用嘴含进满足我视频| 亚洲熟女少妇一区二区三区| 无码中文日本精品一区| 娇妻在客厅被朋友玩得呻吟| 岳肥肉紧嫩嫩伦69| 亚洲成A人无码亚洲成AV无码| 苍井空在线AV播放| 国产FREEXXXX性播放麻豆| 全黄H全肉1V1各种姿势| 欧美重囗味sm群虐视频| 老熟妇高潮一区二区三区| 国产精品毛片一区二区| 性的暴行中文完整版| 国产一产二产三精华液区别在哪里| 国产AⅤ无码专区亚洲AV麻豆| 99精品国产在热久久无码| 亚洲码国产精品高潮在线| 我的师妹不可能是傻白甜| 波多野结衣人妻| 巜疯狂的少妇4做爰| 色综合久久无码五十路人妻| 涩涩AV视频一区二区三区| 日本理论片| 国产L精品国产亚洲区久久| 国产精品无码专区AV在线播放 | 无码国产精品一区二区免费虚拟VR| 山村最原始的肉欲伦情| 欧美电影在线观看| 无码人妻精品一区二区三区夜夜嗨 | 一夜新娘第一季全集免费观看| 日本爆乳强伦中文字幕电影| 亚洲AV无码国产精品久久不卡| 亚洲AⅤ在线无码播放毛片一线天 国产aⅴ激情无码久久久无码 | 欧美疯狂做受XXXX高潮| 国产真实老头老太BBWBBW |