玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>HTB-103KATO III細(xì)胞, 人胃癌細(xì)胞

KATO III細(xì)胞, 人胃癌細(xì)胞

簡(jiǎn)要描述:KATO III細(xì)胞, 人胃癌細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):HTB-103
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問(wèn)  量:1870

產(chǎn)品分類

Product Category

詳細(xì)介紹

KATO III細(xì)胞, 人胃癌細(xì)胞

OrganismHomo sapiens, human
Tissue

stomach:derived from metastatic pleural effusion; supraclavicular and axillary lymph nodes and Douglas cul-de-sac

Product Formatfrozen
Morphologyspherical
Culture Propertiesmixed, adherent and suspension
Biosafety Level1
Diseasegastric carcinoma
Age55 years adult
Gendermale
EthnicityAsian
Storage Conditionsliquid nitrogen vapor phase
KaryotypeThe stemline chromosome number is hypotetraploid with the 2S component occurring at 6.2%. Nine markers were common to most S metaphases, four markers were less frequent. One (occasionally 2 copies) homogenous staining region (HSR) (t(11;HSR) was present in all metaphases examined, but no double minutes (DM) were detected.
Clinical Data

55 years adult

Asian

male

TumorigenicYes
Effects

Yes, in cheek pouches of anti thymocyte serum treated hamsters

No, in nude mice

Complete Growth MediumThe base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
KATO III細(xì)胞, 人胃癌細(xì)胞
Subculturing

Protocol: Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove culture medium with floating cells to a centrifuge tube. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor. 2.. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. 3. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 4. To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximay 125 xg for 5 to10 minutes. 5. Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels. 6. Place culture vessels in incubator at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: 5% CO2 in air recommended

Temperature: 37°C          






















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
暴力肉体进入HDXXXX| 国产亚洲精品久久久久久久久动漫 | 国产欧美精品aaaaaa片| 日本55丰满熟妇厨房伦| 蜜臀av国产精品久久久久 | 亂倫近親相姦中文字幕| 亚洲午夜久久久久久久久电影网| 国产成人AAAAA级毛片| 国精产品W灬源码16| 久久鸭和久久丫是不是一个品牌 | 日产精品一线二线三线芒果| 日本亲与子乱人妻HD| 99久久久无码国产精品9| 久久久受WWW免费人成| 人妻丰满熟妞AV无码区| 亚洲精品国产熟女久久久| 亚洲熟女乱综合一区二区| 久久久久久国产精品免费免费男同| 激情97综合亚洲色婷婷五| 无码人妻精品一区二区三区66 | 大又大又粗又硬又爽少妇毛片| 熟妇熟女乱妇乱女网站| 偷窥日本少妇撒尿com| 成年性生交大片免费看| 狮王的巨大挺进体内H| 久久久久久国产精品MV| 午夜福利理论片在线观看| 国产精品亚洲色婷婷99久久精品| 国产真实的和子乱拍在线观看| 使劲快高潮了国语对白在线| 92久久偷偷做嫩草影院免费看| 美女露0的身体无档软件| 亚洲日韩一区二区三区| 亚洲AV永久一区二区三区| 性熟妇VIDEOS另类HD| 日日噜噜噜噜夜夜爽亚洲精品 | 国产69TV精品久久久久99| 亚洲精华国产精华精华液好用吗| 国产一区二区精品久久岳| 入禽太深全文免费视频| 被多个强壮黑人灌满精H|