玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠正常細(xì)胞>CRL-1772C2C12細(xì)胞, 小鼠成肌細(xì)胞

C2C12細(xì)胞, 小鼠成肌細(xì)胞

簡(jiǎn)要描述:C2C12細(xì)胞, 小鼠成肌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1772
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問(wèn)  量:1929

產(chǎn)品分類

Product Category

詳細(xì)介紹

C2C12細(xì)胞, 小鼠成肌細(xì)胞

ATCC® Number:CRL-1772™    Price:$256.00
Designations:C2C12Biosafety Level:1Shipped:frozenMedium Properties:& Serum:oSee PropagationGrowthadherentOrganism:Mus musculus (muse)Morphology:myoblastSource:Strain: C3H Tissue: muscle Cell Type: myoblast;Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. C2C12細(xì)胞, 小鼠成肌細(xì)胞Applications:transfection host (Nucleofection technology from LonzaRoche FuGENE® Transfection Reagents)Comments:This is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. [22903] The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins. [22953] Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic. [23427] Tested and found negative for ectromelia virus (mousepox).Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°CSubculturing:Protocol: IMPORTANT - DO NOT ALLOW CULTURES TO BECOME CONFLUENT. Cultures must not be allowed to become confluent as this will deplete the myoblastic population in the culture.Myotube formation is enhanced when the medium is supplemented with 10% horse serum instead of fetal bovine serum.Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Inoculate at a cell concentration between 1.5 X 10 exp5 and 1.0 X 10 exp6 viable cells/75 cm2.Incubate cultures at 37°C.



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产V亚洲V天堂无码| 51精品少妇人妻AV一区二区| 麻豆妓女爽爽一区二区三| 欧美+国产+日产| 日韩V亚洲V欧美V精品综合| 亚洲熟女AV中文字幕男人总站| 韩国理仑片色情在线观看| 久久WWW香蕉免费人成| 国产欧美日韩一区二区三区 | 亚洲AV中文无码乱人伦在线R▽| 国产永久免费CRM系统有哪些| 无码AV人妻一区二区三区四区| 两片肥厚乌黑的陰唇| 理科生坠入情网电视剧免费观看| 中文乱码字慕人妻熟女人妻| 打屁股羞耻扒开撅着| 国产精品久久久久久久久免费| 工头搡老女人老妇女老熟女| 小SAO货水好多真紧H无码视频| 黑人粗大猛烈进出高潮视频| 锕锕锕锕锕锕~再深一点五种软件| 自拍偷区亚洲国内自拍| 色综合久久久久综合99| 老熟女高潮一区二区三区| 铁甲二手工程机械网| 国产精品久久久久乳精品爆| 人妻人人做人做人人爱| 精品国产乱码久久久久久郑州公司| 午夜精品A片一区二区三区| 国产黄A三级三级三级看三级| 极品美女扒开粉嫩小泬AV视频| 午夜精品A片一区二区三区| 老男人把舌头伸进我下面| 国产综合精品一区二区三区| 久久久久久久久无码精品亚洲日韩| 欧美人与劲物xxxxz0oz| 无码日韩人妻精品久久蜜桃入口| 国产FREEXXXX性播放麻豆| 日本丰满少妇XXXX| 女公务员人妻呻吟求饶| 成人性做爰AAA片免费看|