玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>HTB-148H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞

H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞

簡(jiǎn)要描述:H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):HTB-148
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問(wèn)  量:1612

產(chǎn)品分類

Product Category

詳細(xì)介紹

H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞

ATCC® Number:HTB-148™    Price:$338.00
Designations:H4Depositors: J RiggsBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: brain Disease: neurogliomaPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞Applications:transfection host (Roche FuGENE® Transfection Reagents)Tumorigenic:NoDNA Profile (STR):Amelogenin: X,Y CSF1PO: 10,12 D13S317: 12 D16S539: 11,12 D5S818: 10,12 D7S820: 8,11 THO1: 7,9 TPOX: 8,11 vWA: 14,18Cytogenetic Analysis:modal number = 73; range = 63 to 78.This is a hypertriploid human cell line having the modal chromosome number of 73 occurring in 26% of cells. However, cells having 75 chromosomes also occurred at a high rate (24%). Higher ploidies were found at 0.4%. At least 16 marker chromosomes are common to all metaphases examined: paired del(2)(p2209) and del(9)(p22) and single der(14)t(1;14)(p22;q22), del(3)(p2501), del(7)(q3209), del(10)(p1301), t(13q17q), t(3p13q) and at least eight others. The del(5) (p13), del(7) (q11) and a few others occurred in some, and many others were seen only once. N7 and N21 occurred in 4 or more copies per cell. Most cells had two X and two Y chromosomes.Isoenzymes:AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 0 PGM1, 1-2 PGM3, 1Age:37 yearsGender:maleEthnicity:CaucasianPropagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°CSubculturing:Protocol:             Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:10 to 1:15 is recommended Medium Renewal: 2 to 3 times per weekPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002recommended serum:ATCC 30-2020References:22287: Arnstein P, et al. Propagation of human tumors in antithymocyte serum-treated mice. J. Natl. Cancer Inst. 52: 71-84, 1974. PubMed:454402622907: Day RS, Ziolkowski CH. Human brain tumour cell strains with deficient host-cell reactivation of N-methyl-N'-nitro-N-nitrosoguanidine-damaged adenovirus 5. Nature 279: 797-799, 1979. PubMed: 450131



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
av无码免费岛国动作片| 女女车车的车车视频免费| 玩弄白嫩少妇XXXXX性| 色欲久久久天天天综合网| 我和岳M愉情XXXX国产| 人妻无码久久一区二区三区免费| 欧美精品九九99久久在免费线| 女人18片毛片60分钟| 国产精品乱码一区二三区| 白雪被出租车司机强好爽| 18VIDEOSEX性欧美69| 国产伦精品一区二区三区免费迷| 国产精品久久久久精品日日| 国产精品久久久久久影视| 337P粉嫩大胆色噜噜噜| 亚洲VA国产VA天堂VA久久| 国产亚洲婷婷香蕉久久精品| 办公室秘书跨坐蹭揉H| 97久久精品亚洲中文字幕无码| 特黄把女人弄爽又大又粗| 精品黑人一区二区三区久久| 国产69精品久久久久人妻| 日韩精品无码一区| 在线免费黄色电影| 中文字幕亚洲乱码熟女在线萌芽| 中国成熟妇女毛茸茸| 艳妇荡女欲乱双飞两中年熟妇| 边啃奶头边躁狠狠躁视频免费观看| 国产无遮挡a片又黄又爽| 国产自拍在线观看| 美国色情三级欧美三级| 人妻激情偷乱一区二区三区| 久久精品午夜一区二区福利| 国产大人和孩做爰BD| 97久久精品人妻人人搡人人玩 | 处破女A片60分钟粉嫩免费观看 | XXX少妇厨房XXX乱| 欧美人与善交大片免费看| 午夜无码熟熟妇丰满人妻| 1000部精品久久久久久久久| 天天爽夜夜爽夜夜爽|