玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>HTB-148H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞

H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞

簡(jiǎn)要描述:H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):HTB-148
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問(wèn)  量:1612

產(chǎn)品分類

Product Category

詳細(xì)介紹

H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞

ATCC® Number:HTB-148™    Price:$338.00
Designations:H4Depositors: J RiggsBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: brain Disease: neurogliomaPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. H4細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞Applications:transfection host (Roche FuGENE® Transfection Reagents)Tumorigenic:NoDNA Profile (STR):Amelogenin: X,Y CSF1PO: 10,12 D13S317: 12 D16S539: 11,12 D5S818: 10,12 D7S820: 8,11 THO1: 7,9 TPOX: 8,11 vWA: 14,18Cytogenetic Analysis:modal number = 73; range = 63 to 78.This is a hypertriploid human cell line having the modal chromosome number of 73 occurring in 26% of cells. However, cells having 75 chromosomes also occurred at a high rate (24%). Higher ploidies were found at 0.4%. At least 16 marker chromosomes are common to all metaphases examined: paired del(2)(p2209) and del(9)(p22) and single der(14)t(1;14)(p22;q22), del(3)(p2501), del(7)(q3209), del(10)(p1301), t(13q17q), t(3p13q) and at least eight others. The del(5) (p13), del(7) (q11) and a few others occurred in some, and many others were seen only once. N7 and N21 occurred in 4 or more copies per cell. Most cells had two X and two Y chromosomes.Isoenzymes:AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 0 PGM1, 1-2 PGM3, 1Age:37 yearsGender:maleEthnicity:CaucasianPropagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°CSubculturing:Protocol:             Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:10 to 1:15 is recommended Medium Renewal: 2 to 3 times per weekPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002recommended serum:ATCC 30-2020References:22287: Arnstein P, et al. Propagation of human tumors in antithymocyte serum-treated mice. J. Natl. Cancer Inst. 52: 71-84, 1974. PubMed:454402622907: Day RS, Ziolkowski CH. Human brain tumour cell strains with deficient host-cell reactivation of N-methyl-N'-nitro-N-nitrosoguanidine-damaged adenovirus 5. Nature 279: 797-799, 1979. PubMed: 450131



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产精品三级在线观看无码| 久久99国产精品久久99| 中文字幕乱码人妻二区三区| 国产成人精品无码免费播放| 扒开双腿猛进入喷水高潮叫声| 99国产揄拍国产精品人妻| 处破初破苞一区二区三区| 精品一区二区三区免费毛片爱 | 国精品无码人妻一区二区三区| 高潮vpswindows国产乱| 国产一区二区在线视频| 国产亲妺妺乱的性视频播放| SAO货撅起你的贱屁股来| 偷玩熟睡醉酒体育壮男GV| A片毛片免费看| 伊人色综合久久天天五月婷| 人妻少妇看A偷人无码精品 | 亚洲最大成人网| 亚洲精品一区三区三区在线观看| 无码视频一区二区三区| 极品美女扒开粉嫩小泬| 国产精品久久一区二区三区蜜桃| 精品无码亚洲一区二区三区毛 | 内射人妻无码色AV天堂| 高中女学生破苞视频免费| 女人被添全过程A一片| 亚洲熟女乱综合一区二区 | yy111111少妇无码理论片| 色精阁久艾草国产WWW| 麻豆传媒直播APP下载| 岳两片蚌肉缓缓张开| 成人电影在线观看| 女主播的YIN荡日子NP| 亚洲国产精品成人无码区| 囗交50个动态图片| 成人区色情综合小说| 欧美黑人乱大交BD| 99RE热这里只有精品视频| 青苹果乐园全网影视大全| 男同gv在线观看免费| 亚洲va欧美va天堂v国产综合|