玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2553Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞

Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞

簡(jiǎn)要描述:Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2553
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問  量:2023

產(chǎn)品分類

Product Category

詳細(xì)介紹

Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞

ATCC® Number:CRL-2553™    Price:$417.00
Designations:Panc 02.03Depositors:EM JaffeeBiosafety Level:1edium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Shipped:frozenMMorphology:epithelial Source:Organ: pancreas Disease: adenocarcinomaCellular Products:cytokeratins 7 and 18 [50655]Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Panc 02.03細(xì)胞, 人胰腺癌細(xì)胞Tumorigenic:YesOncogene:K-ras +Antigen Expression:MHC class I +; MHC class II - [50655]DNA Profile (STR):Amelogenin: X CSF1PO: 11,12 D13S317: 12 D16S539: 11 D5S818: 12,13 D7S820: 9,10 THO1: 6 TPOX: 9,12 vWA: 17Age:70 yearsGender:femaleEthnicity:WhiteComments:Panc 02.03 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma. The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655] The cells have a reported plating efficiency of 100%. [50655]Propagation:ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°CSubculturing:Protocol:                    Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C.Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 daysPreservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phaseDoubling Time:28.2 hrs [50655]Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-2020References:50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久久精品中文字幕麻豆发布| 性色AV夜夜嗨AV浪潮牛牛| 偷偷藏不住小说免费阅读| 亚洲AV无码一区二区三区网址 | 亚洲国产一区二区A毛片| 成品网站W灬源APP免费百度| 色欲色香天天天综合VVV| 亚洲综合国产成人丁香五月激情| 亚洲国产精品久久人人爱 | 日韩精品视频一区二区三区| 天天做夜夜爱爽牛牛视频| SM调教室虐男受调教H打开腿| 日本动漫瀑乳H动漫啪啪免费| 最新69成人国产精品视频免费| 亚洲精品一区二区三区四区五区| 肉乳床欢无码A片A片120秒| 长篇乱肉合集乱500小说| 亲嘴扒胸摸屁股激烈视频 | 欧美高清性色生活片免费观看| 热の综合热の国产中文网| 亚洲国产精品无码专区| 白少洁第1一40章免费阅读| 久久婷婷五月综合97色一本一本| 国产A级三级三级三级| 色欲AV人妻精品一区二区三区| 欧美毛片无码又大又粗黑寡妇| gogogo免费高清日本| 蜜臀AV夜夜澡人人爽人人| 欧美性猛交XXXX黑人猛交| 精品人伦一区二区三区蜜桃牛牛| 精品无人国产偷自产在线| 老熟女五十路乱子交尾中出一区| 天天做天天爱天天爽综合网| FREE性丰满白嫩白嫩的HD| 老熟妇小伙子HD另类| 成人乱码一区二区三区AV| 人妻上司厨房出轨2HD院线| 麻豆E奶女教师国产剧情| 亚洲精品久久无码午夜一区二区| 97人妻精品一区二区三区| 日本三级欧美三级人妇视频黑白配 |