玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人正常細(xì)胞>CRL-2741HBE135-E6E7人支氣管上皮細(xì)胞

HBE135-E6E7人支氣管上皮細(xì)胞

簡要描述:HBE135-E6E7人支氣管上皮細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-2741
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-21
  • 訪  問  量:3089

產(chǎn)品分類

Product Category

詳細(xì)介紹

HBE135-E6E7人支氣管上皮細(xì)胞

細(xì)胞貨期8-10個(gè)工作日

上海復(fù)祥生物提供 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,上有細(xì)胞照片,歡迎各位老師!xiangfbio.


說明書:Volumes used in this protocol are for 75 cm2 flask;             proportionally reduce or increase amount of dissociation medium         for culture vessels of other sizes.


HBE135-E6E7人支氣管上皮細(xì)胞

細(xì)胞貨期8-10個(gè)工作日

上海復(fù)祥生物提供 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,上有細(xì)胞照片,歡迎各位老師!xiangfbio.


說明書:Volumes used in this protocol are for 75 cm2 flask;             proportionally reduce or increase amount of dissociation medium         for culture vessels of other sizes.



細(xì)胞貨期8-10個(gè)工作日

上海復(fù)祥生物提供 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件,上有細(xì)胞照片,歡迎各位老師!xiangfbio.


說明書:Volumes used in this protocol are for 75 cm2 flask;             proportionally reduce or increase amount of dissociation medium         for culture vessels of other sizes.


Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells     under an inverted microscope until       cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping, do not agitate the cells     by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by pipetting gently.

To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 x g for 5 to 10 minutes.

Discard supernatant and resuspend cells in fresh serum-free growth medium.  Add appropriate aliquots of cell suspension to new culture vessels.

Place culture vessels in incubators at 37°C.


Subc*tion Ratio: 1:3 to 1:4

Medium Renewal: Every 2 to 3 days.

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells: a Manual of Basic Technique by R. Ian Freshney, 3th edition, published by Alan R. Liss, N.Y., 1994.

培養(yǎng)條件:Keratinocyte-Serum Free medium with 5 ng/ml human recombinant EGF (do not filter) and 0.05 mg/ml bovine pituitary extract (Invitrogen, formerly GIBCO-BRL, Cat.  17005-042) and supplemented with 0.005 mg/ml insulin and 500 ng/ml hydrocortisone.
























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
少妇XXXXX性开放| 人妻 日韩 欧美 综合 制服 | 同性双男A片又黄又刺激小说免费| 欧美肥婆性猛交XXXX| 小肚子鼓起按H宫交| 诱人的大乳BD在线观看| 99热这里只有精品| 公司办公室C了我好多次| 国产女人18毛片水真多1| 吃了他达拉非太硬还需要继续吃吗| 男人添女人囗交做爰视频| 88国产精品欧美一区二区三区| 欧美AV噜噜狠狠网址蜜桃| 一本色道久久爱久久综合网| 亚洲熟妇无码AV不卡在线播放| 亚洲国产成人精品女人久久久| 国产无遮挡裸体免费视频| 撕开奶罩揉吮奶头玩大胸视频 | 熟妇人妻系列AV无码一区二区| 女浴室里赤裸裸洗澡丰满视频| 熟女人妇 成熟妇女系列视频| 久久无码人妻一区二区三区| 女性私密粉嫩部位| 俄罗斯少妇性做爰片| 无码精品黑人一区二区三区 | 国产亚州精品女人久久久久久| 无码人妻精品一区二区三区东京热| 打开这个网站你会感谢我的| 白丝双马尾被疯狂输出| 国产精品久久久久久一区二区三区| 777久久精品一区二区三区无码| 老公一到晚上就日不停| 939W乳液78W78W韩国| 国内精品人妻无码久久久影院蜜桃| 一边摸一边脱一边吃胸亲| 欧美十八禁激情毛片爱情与灵药| 成人性毛片免费观看黄 | 学生小嫩嫩馒头在线观看| 装醉把自己给流浪汉玩| 久久精品国产亚洲AV高清热| 精品国产产一区二区三区久久|