玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢(xún)熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>EA.hy926人臍靜脈細(xì)胞融合細(xì)胞

人臍靜脈細(xì)胞融合細(xì)胞

簡(jiǎn)要描述:CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞;
細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):EA.hy926
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問(wèn)  量:4325

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹


ATCC® Number: CRL-2922™ Price:

Designations:

EA.hy926

Depositors: CS Edgell

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: endothelial

CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞

Source: Tissue: somatic cell hybrid

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Restrictions: For-profit institutions must obtain license from University of North Carolina, Chapel Hill.

Antigen Expression: Factor VIII-related antigen; Homo sapiens, expressed

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11,12

D13S317: 11

D16S539: 11,12

D5S818: 11

D7S820: 8,9,10

THO1: 6,8,9.3

TPOX: 8,9

vWA: 14,17

CRL-2922 EA.hy926 人臍靜脈細(xì)胞融合細(xì)胞

Comments: The human umbilical vein cell line, EA.hy926, was established by fusing primary human umbilical vein cells with a thioguanine-resistant clone of A549 by exposure to polyethylene glycol (PEG). Hybrid clones were selected in HAT medium and screened for factor VIII-related antigen. EA.hy926 cells have been maintained for more than 100 population doublings (PDLs). Electron photomicrographs demonstrate cytoplasmic distribution of Weibel-Palade bodies and tissue-specific organelles, characteristics of differentiated endothelial cell functions such as angiogenesis, homeostasis/thrombosis, blood pressure and inflammation. [PubMed: 6407019] [PubMed: 2079463]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10.

Atmosphere: air, 95; carbon dioxide (CO2), 5

Temperature: 37.0°C

Subculturing: Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with Ca++/Mg++ free Dulbeccos phosphate-buffered saline (D-PBS) or 0.25 (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

CRL-2922 EA.hy926

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Transfer cell suspension to a centrifuge tube and spin at approximay 125 X g for 5 to 10 minutes. Discard supernatant.

6.Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 X 10(3) to 3 X 10(3) viable cells/sq. cm is recommended.

7.Incubate cultures at 37C. Subculture when cell concentration reaches between 8 X 10(4) and 1 X 10(5) cells/sq. cm.

Interval: Twice a week

Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days


Preservation: Freeze medium: Complete Growth medium, 95; DMSO, 5

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

CRL-2922 EA.hy926

0.25 (w/v) Trypsin - 0.53 mM EDTA in Hank BSS (w/o Ca++, Mg++):ATCC 30-2101

phosphate-buffered saline:ATCC 30-2200

Cell culture tested DMSO:ATCC 4-X

Erythrosin B vital stain solution:ATCC 30-2404

Trypan Blue vital stain solution:ATCC 30-2402

References: 92902: Edgell CJ, et al. Permanent cell line expressing human factor VIII-related antigen established by hybridization. Proc. Natl. Acad. Sci. USA 80: 3734-3737, 1983. PubMed: 6407019

92903: Bauer J, et al. In vitro model of angiogenesis using a human endothelium-derived permanent cell line: contributions of induced gene expression, G-proteins, and integrins. J. Cell. Physiol. 153: 437-449, 1992. PubMed: 1280276

92904: Edgell CJ, et al. Endothelium specific Weibel-Palade bodies in a continuous human cell line, EA.hy926. In Vitro Cell. Dev. Biol. 26: 1167-1172, 1990. PubMed: 2079463

CRL-2922 EA.hy926

92905: Rieber AJ, et al. Extent of differentiated gene expression in the human endothelium-derived EA.hy926 cell line. Thromb. Haemostasis 69: 476-480, 1993. PubMed: 8322270





























產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
永久免费a∨片在线观看| 久久久久久久性潮| 欧美精品乱码99久久蜜桃| 国模精品一区二区三区| 自拍偷自拍亚洲精品播放 | 国产精品日韩欧美一区二区三区| 少妇高h肉辣全集目录| 久久中文字幕人妻熟AV女| 国产成人无码免费看片软件| 亚洲精品无码成人片久久不卡| 少妇被躁爽到高潮无码人狍大战| 老师破女学生处特级毛OOO片| 99久久精品无码一区二区毛片| 老头猛吸女大学奶头A片| 欧美熟妇另类久久久久久不卡| 被闺蜜男朋友强肉H高干| 久久99亚洲网美利坚合众国 | 农村熟女XXXXHD做受| SM调教室虐男受调教H打开腿| 精品国偷自产在线视频| 欧美,日韩,国产,专区| 小SAO货CAO得你舒服吗男男| 女人与拘高清ZOZ0| 欧美最猛黑人XXXXX猛交| 14表妺好紧没带套经过| 69麻豆天美精东蜜桃传媒| 伊人久久大香线蕉AV影院| 娇妻系列交换(纯肉高H)| YIN荡纯肉体育生NP男男| 久久无码AV中文出轨人妻| 娇妻当着我的面被4P| 少妇AV一区二区三区无码| 天天做天天爱夜夜爽毛片毛片| 国产AV成人无码免费视频| 亚洲视频一区| AV无码精品一区二区三区四区 | 欧美成人一区二区三区在线视频| 亚洲av无码一区二区三区在线| 国产特级毛片AAAAAA| 国产精品JIZZ在线观看老狼| 非缘勿扰电视剧全集36免费观看|