玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2557Panc 05.04 人胰腺癌細(xì)胞

Panc 05.04 人胰腺癌細(xì)胞

簡(jiǎn)要描述:CRL-2557 Panc 05.04 人胰腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2557
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問(wèn)  量:1806

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2557 Panc 05.04 人胰腺癌細(xì)胞

ATCC® Number: CRL-2557™ Price: $417.00

Designations: Panc 05.04

Depositors: EM Jaffee

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial

Source: Organ: pancreas

Disease: adenocarcinoma

Cellular Products: cytokeratins 7 and 18 [50655]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2557 Panc 05.04 人胰腺癌細(xì)胞

Isolation: Isolation date: May 5, 1995

Tumorigenic: Yes

Oncogene: K-ras +

Antigen Expression: MHC class I +; MHC class II - [50655]

Blood type B; Rh+

Age: 77 years adult

Gender: female

Ethnicity: White

Comments: Panc 05.04 is a pancreatic adenocarcinoma epithelial cell line derived, in 1995, from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.

The cell line exhibits a K-ras oncogene mutation at codon 12 where a GGT --> GAT mutation resulted in substitution of aspartic acid for glycine. [50655]

The cells have a reported plating efficiency of 100%. [50655]

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 20 Units/ml human recombinant insulin, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:5 is recommended

Medium Renewal: Add media once per week. Fluid change one to two times per week.

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 46 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

CRL-2557


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
久久久久噜噜噜亚洲熟女综合| 99久久精品国产亚洲av| 狠狠躁天天躁夜夜躁婷婷| 女性私密粉嫩部位| 波多野结衣456| 日本强伦姧人妻一区二区| 男男调教羞耻H扒开鞕臀海棠| 一女三黑人玩4P惨叫A片| 久久久久久精品无码人妻| 亚洲AV无码精品国产成人| 久久久久成人精品免费播放动漫| 无码人妻久久一区二区三区不卡 | 色哟哟免费精品网站入口| 精品久久久久久久久久中文字幕| 曰本女人与公GUO交酡视频A片| 性夜影院爽黄a爽免费看不卡| 国产无遮挡裸体免费视频| 少妇高潮惨叫久久久久久久| 迎合疯狂肉伦耕耘播种| 九九久久精品无码专区| 国产女人高潮视频在线观看| 美女黄网站视频免费视频软件| 成人精品视频一区二区三区尤物| 妈妈的朋友4在线观看| 我和公发生了性关系视频| 色视频WWW在线播放国产人成| 亚洲午夜精品一区二区麻豆| 一边摸一边叫床一边爽| 同桌上课脱裙子让我帮他自慰 | 国产乱自产黄A片在线观看| CAOPORN免费视频在线| 色婷婷AV视频一二三区小说| 人妻偷人VA精品国产旡码| 99精品国产高清一区二区麻豆| 无码国产一区二区三区四区公司| 丰满爆乳无码一区二区三区| 国产av国产av在在免费线| 欧美bbbwbbwbbwbbw| av无码免费岛国动作片| 伴郎粗大的内捧猛烈进出第一章| 亚洲V欧美V国产V在线观看|