玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CCL-105SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞

SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞

簡(jiǎn)要描述:CCL-105 SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞,
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,
提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CCL-105
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-12
  • 訪  問(wèn)  量:1746

產(chǎn)品分類

Product Category

詳細(xì)介紹

CCL-105 SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞的介紹

CCL-105 SW-13 人腎上腺皮質(zhì)腺癌細(xì)胞

ATCC® Number: CCL-105™ Price: $323.00

Designations: SW-13

Depositors: A Leibovitz

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: epithelial


Source: Organ: adrenal gland

Tissue: cortex

Tumor Stage: grade IV

Disease: primary small cell carcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Isolation: Isolation date: August, 1971

Virus Susceptibility: Human poliovirus 1

Vesicular stomatitis virus

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 9

D16S539: 12

D5S818: 12

D7S820: 8,10

THO1: 7,8

TPOX: 8

vWA: 17,19

Cytogenetic Analysis: modal number = 63; range = 45 to 65.

Approximay 10% of the cells examined possessed a pair of dicentric chromosomes.

Isoenzymes: G6PD, B

Age: 55 years

Gender: female

Ethnicity: Caucasian

Comments: Electron microscopic studies show many bulb gap junctions (BGJ).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

1.Remove and discard culture medium.

2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

5.Add appropriate aliquots of the cell suspension to new culture vessels.

6.Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020

References: 22140: . . In Vitro 8: 443, 1973.

22526: Leibovitz A, et al. New human cancer cell culture lines. I. SW-13, small-cell carcinoma of the adrenal cortex. J. Natl. Cancer Inst. 51: 691-697, 1973. PubMed: 4765382

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

23212: Lasfargues EY, Ozzello L. C*tion of human breast carcinomas. J. Natl. Cancer Inst. 21: 1131-1147, 1958. PubMed: 13611537

26278: Johnson RG, Sheridan JD. Junctions between cancer cells in culture: ultrastructure and permeability. Science 174: 717-719, 1971. PubMed: 4330805

26279: Pinto da Silva P, Gilula NB. Gap junctions in normal and transformed fibroblasts in culture. Exp. Cell Res. 71: 393-401, 1972. PubMed: 4339896

CCL-105


















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
97视频在线观看| 波多野たの结衣在线播放| 饥渴老翁一晚要了我三次| 看片神器免费| 欧美疯狂做受XXXX高潮| 亚洲日韩AV无码精品放毛片| 偷窥23个美女撒尿视频| 黄海川邱舒涵全文免费阅读| 久久久久无码专区亚洲AV| 亚洲va欧美va天堂v国产综合| 欧美iphonexsmax免费| 久久久久久亚洲精品不卡| 久久99精品国产自在现线小黄鸭| 99国产精品国产精品九九 | 国产麻豆精品一区二区三区V视界| 亚洲日本无码一区二区三区四区卡 | АⅤ资源天堂资源库在线| 同桌上课脱裙子让我帮他自慰| 亚洲日韩精品AV成人波多野| 国产精品国色综合久久| 色欲精品国产一区二区三区AV| 国模雨珍浓密毛大尺度150P| 成年美女黄网站色大免费视频| 国产精品色欲AV亚洲三区小说| 女子初尝黑人巨嗷嗷叫 | 色欲AV亚洲波多野结衣| 最近中文字幕高清字幕mv| 草莓视频免费观看| 中文字幕乱码中文字乱码| 国产精品久久久久久人妻| hkdoll玩偶在线无码国产| 姓一乱一口一交A片文| 涩涩AV视频一区二区三区| 国自产拍偷拍精品啪啪一区二区 | 国产边添边摸边做边爱| 少妇洁白无删减版178TXT| 日产无人区一线二线三线 | 奇米影视7777久久精品| 成人电影在线观看| 日韩国产精品无码一区二区三区| 被夫の上司持久侵犯耻辱在线|