玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢(xún)熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>小鼠正常細(xì)胞>CRL-1772C2C12 小鼠成肌細(xì)胞

C2C12 小鼠成肌細(xì)胞

簡(jiǎn)要描述:CRL-1772 C2C12 小鼠成肌細(xì)胞,
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|;
細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-1772
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問(wèn)  量:2226

產(chǎn)品分類(lèi)

Product Category

詳細(xì)介紹

CRL-1772 C2C12 小鼠成肌細(xì)胞的詳細(xì)介紹

CRL-1772 C2C12 小鼠成肌細(xì)胞

ATCC® Number:CRL-1772™  Price:$256.00
Designations:C2C12

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:myoblast
C2C12 小鼠成肌細(xì)胞


Source:Strain: C3H
Tissue: muscle
Cell Type: myoblast;


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this . AATCC materialnyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-1772

Applications:transfection host (Nucleofection technology from Lonza

Roche FuGENE® Transfection Reagents)



Comments:This is a subclone (produced by H. Blau, et al) of the mouse myoblast cell line established by D. Yaffe and O. Saxel. [22903]

The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins. [22953]

Treatment with bone morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic. [23427]

Tested and found negative for ectromelia virus (mousepox).



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Protocol: IMPORTANT - DO NOT ALLOW CULTURES TO BECOME CONFLUENT.

Cultures must not be allowed to become confluent as this will deplete the myoblastic population in the culture.

Myotube formation is enhanced when the medium is supplemented with 10% horse serum instead of fetal bovine serum.

  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

    Inoculate at a cell concentration between 1.5 X 10 exp5 and 1.0 X 10 exp6 viable cells/75 cm2.

  6. Incubate cultures at 37°C.




















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
伊人色综合久久天天伊人| 少妇又色又紧又爽又刺激视频| 嗯灬啊灬把腿张开灬A片| 好男人资源在线看免费的| 办公室欧美大尺寸suv| 色婷婷香蕉在线一区二区| 成AV免费大片黄在线观看| 国产精品久久久久久无码专区| 第一次进去丫头的身体怎么办| 精精国产XXXX视频在线播放| 男男强行扒开小受双腿进入| 无码人妻精品一区二区三区99| A性色生活片久久毛片牛牛| 哦┅┅快┅┅用力啊┅警花少妇| 三妻四妾免费观看完整版高清| 欧美黑人乱大交| 蜜芽va亚洲va欧美va天堂 | 齐天大性之大闹盘丝洞| 欧美交换配乱吟粗大| 欧美精品一区二区三区在线观看| 精品视频无码一区二区三区| 中国成熟妇女毛茸茸| 99精品国产在热久久婷婷| 亚洲国产精华液2020| 无码AV免费一区二区三区试看| 国产自拍av| 人与鲁专区VR| 少妇少洁第1一40章| 久久久久久国产精品免费免费| 精品人伦一区二区三区蜜桃免费 | 欧美亚洲一区二区三区| 99久久久免费精品国产| 白少洁第1一40章免费阅读| 又黄又爽又猛1000部A片| 亚欧免费无码AⅤ在线观看| 饭桌上故意张开腿让公| 夜夜爽妓女8888视频免费观看| 亚洲精品无码久久久久秋霞| 国产精品无圣光一区二区| 国产精品VA最新国产精品视频| 免费看美女被靠到爽的视频|