玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠腫瘤細(xì)胞、癌細(xì)胞>CRL-6475B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞

B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞

簡要描述:CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號:CRL-6475
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2068

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-6475 B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞

ATCC® Number:CRL-6475™  Price:$269.00
Designations:B16-F10

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:melanocyte
B16F10 小鼠黑色素瘤高轉(zhuǎn)移細(xì)胞


Source:Organ: skin
Strain: C57BL/6J
Disease: melanoma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-6475

Applications:transfection host (technology from amaxa)

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


    1. Subc*tion Ratio: A subc*tion ratio of 1:10 is recommended
      Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase



CRL-6475
















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
交换俱乐部娇妻奶呻吟啊视频| 日产无码久久久久久精品| 亚洲AV无码国产精品色| 97无码欧美熟妇人妻蜜桃天美| 国产成人av| 日本双人XXOO吃奶视频| 国产卡一卡二卡三乱码手机| 偷窥23个美女撒尿视频| 人妻少妇被粗大爽9797PW| 老师露双奶头无遮挡挤奶视频| 偷窥日本少妇撒尿com| ASS白嫩白嫩的少妇PICS| 亚洲一区二区三区女厕偷拍| 成人毛片18女人毛片免费看视频| 精品久久久久久亚洲综合网| 黑人巨大三根一起进| 97无码精品人妻一区二区| 成人电影在线播放| ass鲜嫩鲜嫩pics日本| 无码人妻精品一区二区三区不卡| 寡妇两腿间黑黑的毛| 国产亚洲精品久久久久秋霞| 70岁老太把腿岔开给老头摸| 97视频在线观看| 欧美人与兽| 久久蜜桃精品一区二区三区| 欧美军警gay巨大粗长| 免费看真人直播| 亚洲AV无码一区二区三区DV| 国产精品99久久久久久人| 亚洲综合AV一区二区三区| 无人高清视频完整版在线观看| 99精品人妻无码专区在线视频区| 免费看成人做xxoo视频| 宝贝帮我拉开拉链它想你了| 羞羞漫画_成人漫画_成人专用| 国产真实伦对白全集| 色狠狠久久AV五月丁香| 岳的大肥屁熟妇五十路99| 亚洲性色精品一区二区在线| A片做爰片仑理片免费看|