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M-NFS-60 小鼠白血病細胞G-CSF依賴性

簡要描述:CRL-1838 M-NFS-60 小鼠白血病細胞G-CSF依賴性,
ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,
提供的細胞株背景清楚,提供參考文獻和培養(yǎng)條件,

  • 產(chǎn)品型號:CRL-1838
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
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CRL-1838 M-NFS-60 小鼠白血病細胞G-CSF依賴性, ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻優(yōu)培養(yǎng)條件,

CRL-1838 M-NFS-60 小鼠白血病細胞G-CSF依賴性 的詳細介紹

CRL-1838

ATCC® Number:CRL-1838™  Price:$349.00
Designations:M-NFS-60

Depositors:JF Weaver, A Creasey

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:suspension

Organism:Mus musculus (mouse)

Morphology:lymphoblast


Source:Disease: myelogenous leukemia
Cell Type: virus induced


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Applications:transfection host (Roche FuGENE® Transfection Reagents)

Comments:The M-NFS-60 cell line was derived from a myelogenous leukemia induced with the Cas-Br-MuLV wild mouse ecotropic retrovirus.

The cells are responsive to both interleukin 3 (interleukin-3, IL-3) and macrophage colony stimulating factor (M-CSF).

The cells contain a truncated c-myb proto - oncogene caused by integration of a retrovirus.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
  • 0.05 mM 2-mercaptoethanol

  • 62 ng/ml human recombinant macrophage colony stimulating factor (M-CSF)

  • fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0°C



Subculturing:Medium Renewal: Add fresh medium at the time of subculture

Cultures can be maintained by addition or replacement of fresh medium. Subcuture every two days at 2.5 X 10 exp4 viable cells/ml.



Preservation:culture medium 95%; DMSO, 5%

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020



References:22842: Nakoinz I, et al. Differentiation of the IL-3-dependent NFS-60 cell line and adaption to growth in macrophage colony-stimulating factor. J. Immunol. 145: 860-864, 1990. PubMed: 2142710

23295: Weinstein Y, et al. Truncation of the c-myb gene by a retroviral integration in an interleukin 3-dependent myeloid leukemia cell line. Proc. Natl. Acad. Sci. USA 83: 5010-5014, 1986. PubMed: 3014527



















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