玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠腫瘤細(xì)胞、癌細(xì)胞>CRL-2541C8-D1A 小鼠小腦細(xì)胞

C8-D1A 小鼠小腦細(xì)胞

簡要描述:CRL-2541 C8-D1A 小鼠小腦細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號:CRL-2541
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2656

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2541 C8-D1A 小鼠小腦細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件


CRL-2541 C8-D1A 小鼠小腦細(xì)胞

ATCC® Number: CRL-2541™    Price: $339.00
Designations: C8-D1A [Astrocyte type I clone]

Depositors: B Pessac, D Trisler

Biosafety Level:1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:neuronal


Source:Organ: brain
Strain: C57BL/6
Tissue: cerebellum


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-2541

Cytogenetic Analysis:pseudodiploid, pseudodiploid [48963]

Age: 8 days

Comments:Clonal permanent cell lines with astrocytic or microglial properties have been established from explant cultures of 8-day postnatal mouse cerebella after in vitro spontaneous transformation. [48963] [49701]

Sister flasks of lethally irradiated astrocytes, which are known to synthesize the macrophage-microglia growth factor, M-CSF, were used as a substrate for cloning the cells. [48963] [49701]

The cell lines were derived in a multistage process. Slowly proliferating foci with several morphologies appeared 4 months after initiation of the cultures and became progressively enriched by cells with a homogeneous appearance. [48963]

Some of these cloned cell lines bound anti-glial fibrillary acidic protein (GFAP) antibodies and therefore appeared to be astrocytic. No other glial neuronal or microglial markers have been detected in these clones. [48963]

According to their morphology, 3 separate types of these GFAP-positive clones could be distinguished. Type I and II cells had small somata. [48963]

Type I had several short processes, while type II had two processes, one of which was very thin and long (greater than 200 microns). Type III cells had large flat somata and no processes. [48963]

The astrocyte type II cloned cell line named C8-S is available as ATCC CRL-2535 and the astrocyte type III cloned cell line named C8-D30 is available as ATCC CRL-2534.

One clone with microglial properties named C8-B4 is available as ATCC CRL-2540.

The C8-D1A cell line has the morphology of fibrous astrocytes.

These astrocytic clones might be the in vitro counterparts of fibrous (type I), or velamentous (type III) astrocytes and of Golgi epithelial cells (type II). [48963]



Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%


Subculturing: Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.


  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days


Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020



References:48963: Alliot F, Pessac B. Astrocytic cell clones derived from established cultures of 8-day postnatal mouse cerebella. Brain Res. 306: 283-291, 1984. PubMed: 6466977

49701: Alliot F, et al. A spontaneously immortalized mouse microglial cell line expressing CD4. Brain Res. Dev. Brain Res. 95: 140-143, 1996. PubMed: 8873987




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
极品美女扒开粉嫩小泬AV视频| 国产一二三精品无码不卡日本| 欧美在线精品一区二区三区不卡| 久久久精品人妻无码专区不卡| 丰满的已婚女人HD中字| 国产精品久久久久久吹潮| 波多野结衣办公室性XXX| 99国产揄拍国产精品人妻| 日本丰满少妇XXXX| 无码人妻AⅤ一区二区三区| 一本加勒比HEZYO无码专区| 久久精品一区二区三区AV| 人妻少妇偷人精品无码| 富婆被躁30分钟黑人大战| 久久精品无码一区二区无码| 揉捏奶头呻吟公交车少妇| gogogo免费高清日本| 久久精品亚洲AV无码乱码三区| 国模精品一区二区三区| 成品网站W灬源码1688特点| 少妇一晚三次一区二区三区| 高中女学生破苞视频免费| 欧美另类vivox21老少配 | 肥臀熟女一区二区三区| 歪歪漫画网免费版在线观看| 被群CAO的合不拢腿H纯肉视频| 成人性生交XXXXX无码| 天码AV无码一区二区三区四区| CHINESE性老妇老女人| 成人片黄网站色大片免费| 99er热精品视频国产免费| 军人全身脱精光自慰| 供人泄欲玩弄的妓女H| 蜜芽796.COO永不失联| 两根撑到极致哭着求饶H| 精品人妻人人做人人爽夜夜爽 | 人妻丰满AV无码中文字幕| 久久久久亚洲AV成人人电影| 疯狂揉肉蒂高潮H失禁视频| 啊灬啊灬啊灬免费毛片| 一边吃奶一边添P好爽视频|