无码人妻少妇色欲AV一区二区,成人区人妻精品一区二区不卡视频 ,很黄的吸乳A片三男一女,亚洲成A人V欧美综合天堂麻豆

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>大鼠正常細(xì)胞>CRL-1589 IEC-18 大鼠回腸細(xì)胞

CRL-1589 IEC-18 大鼠回腸細(xì)胞

簡要描述:CRL-1589 IEC-18 大鼠回腸細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:1854

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

CRL-1589 IEC-18 大鼠回腸細(xì)胞, ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)i優(yōu)培養(yǎng)條件

CRL-1589 IEC-18 大鼠回腸細(xì)胞 的詳細(xì)介紹


ATCC® Number:CRL-1589™  Price:$329.00
Designations:IEC-18

Depositors:A Quaroni

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Rattus norvegicus (rat)

Morphology:epithelial


Source:Organ: ileum
Cell Type: epithelial


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Propagation:ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 Unit/ml bovine insulin, 95%; fetal bovine serum, 5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week


Preservation:Freeze medium: culture medium 82.5%; fetal bovine serum 10%; DMSO 7.5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X



References:22491: Quaroni A, et al. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J. Cell Biol. 80: 248-265, 1979. PubMed: 88453

26096: Quaroni A, Isselbacher KJ. Cytotoxic effects and metabolism of benzo[a]pyrene and 7,12- dimethylbenz[a]anthracene in duodenal and ileal epithelial cell cultures. J. Natl. Cancer Inst. 67: 1353-1362, 1981. PubMed: 6273638





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
边做饭边被躁国产| 上司揉捏人妻丰满双乳电影 | 亚洲AV无码久久寂寞少妇| 亚洲AV无码国产精品久久| 四虎永久在线精品无码| 又小又紧女MAGNET| 同性男男黄G片免费网站18禁| 国产成人精品一区二三区在线观看| 人人澡人人透人人爽| 国产日韩一区二区三免费高清| 亚洲成AV人片在WWW色猫咪| 日本熟妇人妻中出| 999ZYZ玖玖资源站永久无码| 人妻放荡乱绿帽H文| 性生生活20分钟免费| 精品人伦一区二区三区蜜桃免费| 天堂√最新版中文在线| 国产女人AAA级久久久级| 破了亲妺妺的处免费视频国产 | 精品熟人妻一区二区三区四区不卡 | 国产特级毛片AAAAAA高清| 丰满熟妇人妻AV无码区| 中文字幕人妻丝袜二区| 中文无码精品一区二区三区 | 老熟女高潮一区二区三区| 亚洲国产一二三精品无码| 欧美精品九九99久久在免费线 | B站禁止转播404入口| 老地方在线观看免费视频社区| 天天摸夜夜摸摸到高潮 | 在免费JIZZJIZZ在线播放| 欧美亚洲精品SUV| 国产精品久久久久免费A∨| 被男狂揉吃奶胸60分钟视频| 国产精品福利一区二区| 校花的凌羞辱日记高H女女| 97午夜理论片在线影院| 亚洲精品一区中文字幕乱码 | 免费无码又爽又刺激高潮的APP | 妺妺窝人体色WWW在线观看| 亚洲国产精品无码中文在线|