玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>動(dòng)物卵巢細(xì)胞>CRL-9618CHO-K1 倉鼠卵巢細(xì)胞亞株

CHO-K1 倉鼠卵巢細(xì)胞亞株

簡要描述:CRL-9618 CHO-K1 倉鼠卵巢細(xì)胞亞株,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-9618
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:2462

產(chǎn)品分類

Product Category

詳細(xì)介紹

CHO-K1 倉鼠卵巢細(xì)胞亞株

1957年,Puck TT從成年中國倉鼠卵巢的活檢組織建立了CHO細(xì)胞,CHO-K1是CHO的一個(gè)亞克隆。CHO-K1的生長需要

培養(yǎng)條件:F12K培養(yǎng)基(SIGMA,貨號(hào)N3520,添加NaHCO3 2.5g/L),90%;優(yōu)質(zhì)胎牛血清,10%


動(dòng)物種別:中國倉鼠

性別:雌

組織來源:卵巢

形態(tài):上皮細(xì)胞

傳代方法的介紹   1:4-1:8

支原體檢測   陰性

以下是此細(xì)胞ATCC

CHO-K1 (ATCC® CCL-61™)
CHO-K1 倉鼠卵巢細(xì)胞亞株

Organism Cricetulus griseus, hamster, Chinese

Tissue  ovary

Product Format  frozen

Morphology  epithelial-like

Culture Properties  adherent

Biosafety Level  1

Gender  female

Applications

This cell line is suitable as a transfection host.

Storage Conditions  liquid nitrogen vapor phase

Karyotype  Chromosome Frequency Distribution 50 Cells: 2n = 22. Stemline number is hypodiploid.

Derivation

The CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957.

Clinical Data

female

Virus Susceptibility  Vesicular stomatitis, Orsay (Indiana)

Vesicular stomatitis, Glasgow (Indiana)

Getah virus

Virus Resistance

poliovirus 2; modoc virus; Button Willow virus

Comments

The cells require proline in the medium for growth.

Complete Growth Medium  The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w

) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended

Medium Renewal: Once or twice between subculture

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产美女久久精品香蕉69| 老人玩小处雌女视频| 色噜噜狠狠一区二区三区 | 国产400部AV国片免费| 中文字幕AV无码免费一区| 亚洲精品无码一区二区卧室| 久久久久久亚洲精品| 成人做爰高潮A片免费视频| 精品深夜AV无码一区二区| 和上司出差被内谢在线播放| 亚洲AV无码乱码国产精品| 哦┅┅快┅┅用力啊┅警花少妇| 人妻出轨合集500篇最新章节| 中文乱码人妻系列一区二区| 成人做爰高潮A片免费视频| 同桌上课脱裙子让我帮他自慰 | 男男调教惩罚做到哭PLAY| 高清无码一区二区在线观看吞精| 端庄美艳人妻教师的沉沦| 九九精品国产亚洲AV日韩| 国产肥熟女视频一区二区三区| 公交车上~嗯啊被高潮| 黑人巨大vs日本人优在线| 久久久国产打桩机| 日日躁夜夜躁夜夜揉人人视频| 国精品人妻无码一区二区三区牛牛 | 中文字幕AV人妻少妇一区二区| JAPANESE高潮流白浆| 亚洲欧洲精品a片久久99| 无码人妻AV一区二区三区蜜臀| 囗交50个动态图片| av无码免费岛国动作片| 日韩精品福利片午夜免费观着| 久久久久久久久久久精品尤物| 99re8在这里只有精品2| 亚洲精品久久久久中文字幕二区| 久久啪久久全部精品| 妓女嫖客叫床粗话对白| 最美情侣韩国片免费观看| 公交车上拨开丁字裤进入电影| 成全视频在线观看免费高清版|