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當(dāng)前位置:首頁 >產(chǎn)品中心>細胞庫>小鼠腫瘤細胞、癌細胞>CRL-1708QT6 鵪鶉纖維肉瘤細胞

QT6 鵪鶉纖維肉瘤細胞

簡要描述:CRL-1708 QT6 鵪鶉纖維肉瘤細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件

  • 產(chǎn)品型號:CRL-1708
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2024-11-14
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CRL-1708 QT6 鵪鶉纖維肉瘤細胞的詳細介紹

CRL-1708 QT6 鵪鶉纖維肉瘤細胞

ATCC® Number:CRL-1708™  Price:$438.00
Designations:QT6

Depositors:C Moscovici

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Coturnix coturnix japonica (quail, Japanese)

Morphology:fibroblast


Source:Disease: fibrosarcoma
Cell Type: methylcholanthrene induced


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Applications:transfection host (Roche FuGENE® Transfection Reagents)

Virus Susceptibility:Rous sarcoma virus

Avian encephalomyelitis virus

Rabies virus

Marek's disease virus



Tumorigenic:Yes

Propagation:ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 10% tryptose phosphate broth and 5% bovine calf serum
Temperature: 37.0°C


Subculturing:Protocol: Resuspend cells by pipetting fresh medium across the monolayer. Cells may also be dislodged by hitting culture flask against the palm of the hand. Subculture at approximay 75% confluency. Use of 0.5% polyvinylpyrrolidone (PVP) in atrypsin-EDTA solution increases the viability when subculturing by enzymatic means. Neutralize and centrifuge to remove additive.Note: When subcultures are prepared using pipetting or mechanical means thecells adhere lightly to the flask substrate and exhibit a tendency toward clumping.
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week


Preservation:Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2030



References:22385: Moscovici C, et al. Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail. Cell 11: 95-103, 1977. PubMed: 194709

CRL-1708


















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