无码人妻少妇色欲AV一区二区,成人区人妻精品一区二区不卡视频 ,很黄的吸乳A片三男一女,亚洲成A人V欧美综合天堂麻豆

咨詢(xún)熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>動(dòng)物腎細(xì)胞>CRL-1650COS-1 非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞

COS-1 非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞

簡(jiǎn)要描述:CRL-1650 COS-1 非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-1650
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問(wèn)  量:1790

產(chǎn)品分類(lèi)

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

CRL-1650  COS-1 非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件


CRL-1650  COS-1 非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞 的詳細(xì)介紹


ATCC® Number:  CRL-1650™

Designations:  COS-1

Depositors:   Y Gluzman

Biosafety Level: 2 [Cells Contain PAPOVAVIRUS ]

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Cercopithecus aethiops

Morphology: fibroblast


Source: Organ: kidney

Cell Type: SV40 transformed

Cellular Products: T antigen

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host (Nucleofection technology from Lonza

Roche FuGENE® Transfection Reagents)

Virus Susceptibility: SV40 (lytic growth); SV40 tsA209 at 40C; SV40 mutants with deletions in the early region

Comments: This is an African green monkey kidney fibroblast-like cell line suitable for transfection by vectors requiring expression of SV40 T antigen. This line contains T antigen, retains complete permissiveness for lytic growth of SV40, supports the replication of ts A209 virus at 40C, and supports the replication of pure populations of SV40 mutants with deletions in the early region.The line was derived from the CV-1 cell line (ATCC ® CCL-70?) by transformation with an origin defective mutant of SV40 which codes for wild type T antigen. The cells contain a single integrated copy of the complete early region of the SV40 genome.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually 5 to 10 min).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detatch. Cells that are difficult to detatch may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week


Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

parental cell line:ATCC CCL-70

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X

References: 1822: Gluzman Y. SV40-transformed simian cells support the replication of early SV40 mutants. Cell 23: 175-182, 1981. PubMed: 6260373

32348: Mansky LM. The mutation rate of human immunodeficiency virus type 1 is influenced by the vpr gene. Virology 222: 391-400, 1996. PubMed: 8806523

32368: Churchill MJ, et al. The rev-responsive element negatively regulates human immunodeficiency virus type 1 env mRNA expression in primate cells. J. Virol. 70: 5786-5790, 1996. PubMed: 8709194

32373: Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260

32555: Suss-Toby E, et al. Toxoplasma invasion: the parasitophorous vacuole is formed from host cell plasma membrane and pinches off via a fission pore. Proc. Natl. Acad. Sci. USA 93: 8413-8418, 1996. PubMed: 8710885

32582: Chang K, Pastan I. Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996. PubMed: 8552591

32788: Lu FM, Lux SE. Constitutively active human notch 1 binds to the transcription factor CBF1 and stimulates transcription through a promoter containing a CBF1-responsive element. Proc. Natl. Acad. Sci. USA 93: 5663-5667, 1996. PubMed: 8643633

32972: Bhattacharyya DK, et al. Involvement of arginine 120, glutamate 524, and tyrosine 355 in the binding of arachidonate and 2-phenylpropionic acid inhibitors to the cyclooxygenase active site of ovine prostaglandin endoperoxide H synthase-1. J. Biol. Chem. 271: 2179-2184, 1996. PubMed: 8567676

33048: Feng XH, Derynck R. Ligand-independent activation of transforming growth factor (TGF) beta-signaling pathways by heteromeric cytoplasmic domains of TGF-beta receptors. J. Biol. Chem. 271: 13123-13129, 1996. PubMed: 8662796

33149: Wang LH, et al. Identification of thromboxane A2 synthase active site residues by molecular modeling-guided site-directed mutagenesis. J. Biol. Chem. 271: 19970-19975, 1996. PubMed: 8702713

33176: Almaula N, et al. Mapping the binding site pocket of the serotonin 5-hydroxytryptamine 2A receptor. J. Biol. Chem. 271: 14672-14675, 1996. PubMed: 8663249



CRL-1650  



















產(chǎn)品咨詢(xún)

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢(xún)熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢(xún)
  • 掃一掃 微信咨詢(xún)

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
天天爽夜夜爽人人爽,qc| 无码熟熟妇丰满人妻啪啪| 大号BBWASSBIGAV女| 国产精品VA无码一区二区| AV无码小缝喷白浆在线观看| 少洁白妇无删减全文阅读| 狠狠97人人婷婷五月| 性国产SE╳O色欲A片欢迎观看| 大陆三级经典三级在线| 小雪你的奶好大把腿张开| 性色AV蜜臀AV色欲AV| 好男人视频在线观看免费完整版| 解开人妻的裙子猛烈进入| 美女100%裸体无遮挡| 扒开双腿猛进入JK校花免费网站| 天天躁日日躁狠狠躁| 国产精品成人一区二区三区无码| 欧美精品九九99久久在免费线| 久久AV喷吹AV高潮AV欧美| 给我免费播放片高清在线观看| 欧美性猛交XXXX富婆| 日本少妇无码精品12P| 精品欧洲AV无码一区二区| 久久人做人爽一区二区三区| 日本进口DVD播放器多少钱一部| 入戏太深动漫未删减在线观看| 欧洲无线乱码2021| 中文字幕在线无码一区二区三区| 曰本性L交片免费看| 性色AV无码久久一区二区三区 | 偷看18美女洗澡过程| CAOPORN成人免费公开| 天干天干天啪啪夜爽爽AV| 中文人妻熟妇乱又伦精品| 亚洲国产精品成人麻豆| 国产SUV精品一区二区四| 又粗又爽高潮午夜免费视频| 亚洲AV无码精品色午夜APP| 从书房一路做到阳台| 真实的和子乱拍视频| 偷偷色噜狠狠狠狠的777米奇|