玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當前位置:首頁 >產(chǎn)品中心>細胞庫>動物上皮細胞>CCL-64Mv.1.Lu(NBL-7) 貂肺上皮細胞

Mv.1.Lu(NBL-7) 貂肺上皮細胞

簡要描述:CCL-64 Mv.1.Lu(NBL-7) 貂肺上皮細胞
ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件
上海復祥生物公司現(xiàn)貨供應美國典型培養(yǎng)物保藏中心(ATCC)各類細胞,*。 手機 xiangfbio.歡迎各位老師來電咨!

  • 產(chǎn)品型號:CCL-64
  • 廠商性質:生產(chǎn)廠家
  • 更新時間:2024-11-14
  • 訪  問  量:2852

產(chǎn)品分類

Product Category

詳細介紹

CCL-64 Mv.1.Lu(NBL-7) 貂肺上皮細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻優(yōu)培養(yǎng)條件

CCL-64 Mv.1.Lu(NBL-7) 貂肺上皮細胞 的詳細介紹

CCL-64

ATCC® Number:CCL-64™  Price:$275.00
Designations:Mv 1 Lu (NBL-7)

Depositors:AJ Kniazeff

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Musa vison (mink)

Morphology:epithelial


Source:Organ: lung

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Isolation:Isolation date: May, 1964

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Virus Resistance:adenovirus 5; coxsackievirus A9, B5; poliovirus 2

Cytogenetic Analysis:Both male and female diploid cells as well as pseudodiploid cells are present. Approximay 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population., Both male and female diploid cells as well as pseudodiploid cells are present. Approximay 58% of the cells have a chromosome number within + or - 1 of the diploid and one dicentric chromosome is present in some cells of the population.

Age:near term fetus

Gender:male and female mixed

Comments:The Mv 1 Lu (NBL-7) cell line was initiated by A.J. Kniazeff, W.A. Nelson-Rees and N.B. Darby, Jr., in May, 1964, from trypsinized lungs of several nearly full-term, unsexed fetuses of the Aleutian mink.The cells are useful for focus forming assays for murine and feline sarcoma viruses [PubMed: 4366800].

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

purified DNA:ATCC CCL-64D

0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101

Cell culture tested DMSO:ATCC 4-X



References:

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細地址:

  • 補充說明:

  • 驗證碼:

    請輸入計算結果(填寫阿拉伯數(shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術支持:化工儀器網(wǎng)    管理登陸
69丰满少妇AV无码区| 精品久久久久久亚洲综合网 | 国产一区二区在线| 久久亚洲AV无码西西人体| 性熟妇VIDEOS另类HD| 成人午夜精品无码区久久| 图片区小说区另类春色首页| 国产特级毛片AAAAAA毛片| 国产麻豆精品一区二区三区V视界 精品国产黑色丝袜高跟鞋 | 间谍过家家第二季在线观看免费| 国产午夜AV无码无片久久96| 女人18毛片A片久久18软件| 久久午夜夜伦鲁鲁片免费无码影视| 部长侵犯部下属人妻A片| 无限看片的视频高清在线| 性欧美XXXXX乱极品少妇| 国产黄A三级三级三级看三级| 麻豆乱码国产一区二区三区| HD女人奶水授乳MILK| 偷拍激情视频一区二区三区 | 和上司出差被内谢在线播放| 扒开粉嫩小泬舌头伸进去视频| 久久久久亚洲av成人无码电影| 欧美freesex黑人又粗又大| 国产我和亲妺作爱视频| 男男19禁啪啪无遮挡免费| 中国帅气体育生GARY视频| 免费视频完整版软件下载| 永久免费观看不收费的软件5| 久久人人爽人人爽人人AV东京热| 精品人妻系列无码一区二区三区 | 亚洲欧美日韩在线不卡| 4438全国大成网人网站| 亚洲成AV人片在线天堂无| 少妇口述与子做过爱| 精品国产乱码久久久久久免费| 男男潮喷翻白眼漏尿高H漫画| 美女做爰A片毛片AAAA| 国产一区二区在线| 久久99精品国产99久久6尤物| 亚洲AV人人澡人人爽人人夜夜|