玩弄秘书的奶又大又软,99香蕉国产精品偷在线观看,日本japanese熟睡人妻,99香蕉国产精品偷在线观看

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞

CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞

簡(jiǎn)要描述:CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|。細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件!

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問(wèn)  量:2209

產(chǎn)品分類

Product Category

詳細(xì)介紹


CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞


ATCC® Number:CRL-2268™Price:$329.00
Designations:BE(2)-C

Depositors:JL Biedler

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:neuroblast
CRL-2268 BE(2)-C 人神經(jīng)母細(xì)胞瘤細(xì)胞


Source:Organ: brain

Disease: neuroblastoma

Derived from metastatic site: bone marrow



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Restrictions:BE(2)-C was deposited at the ATCC by June L. Biedler, Memorial Sloan-Kettering Cancer Center. BE(2)C is distributed for academic research purposes only. Memorial Sloan-Kettering releases the line subject to the following: 1.)BE(2)-C or its products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of BE(2)-C including any use by a for-profit entity must first be negotiated with Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.

Isolation:Isolation date: November, 1972

DNA Profile (STR):Amelogenin: X

CSF1PO: 10

D13S317: 11

D16S539: 9,11

D5S818: 12

D7S820: 9,10

THO1: 6

TPOX: 11

vWA: 18



Age:2 years

Gender:male

Comments:BE(2)-C is a clone of the SK-N-BE(2) neuroblastoma cell line (see ATCC CRL-2271) that was established in November of 1972 from a bone marrow biopsy taken from child with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy.

BE(2)-C cells have a reported saturation density of greater than 5 X 10 exp5 cells/sq cm.

The cells grow as clusters of flattened neuroblastic cells with occasional fine cell processes (neurites).

Unlike the parent line, they generally do not detach and float.



Propagation:ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C



Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:6 is recommended

Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase



Doubling Time:18 hrs

Related Products:recommended serum:ATCC 30-2020

parental cell line:ATCC CRL-2271























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
亚洲av夜夜欢一区二区三区| 农民工嫖妓50岁老熟女| 女人张开腿让男桶喷水高潮| 人人澡超碰碰97碰碰碰| 夜夜爽一区二区三区精品| 久久久久亚洲AV无码专区电影| 亚洲成A人无码亚洲成AV无码| 欧美日韩中文国产一区发布| 国产精品女A片爽视频爽| 国产肥熟女视频一区二区| 久久变态刺激另类sm按摩| 狠狠88综合久久久久综合网| 12—13女人做A片| 久久WWW香蕉免费人成| 久艾草久久综合精品无码国产 | 久久女婷五月综合色啪小说| 无码国产精品一区二区免费式影视 | 精品无码亚洲一区二区三区毛 | 天美传媒mv免费观看| 亚洲欧美强伦一区二区| 精品国产一二三产品区别在哪| 国内少妇毛片视频| 色吊丝AV中文字幕| 黑人玩弄出轨人妻松雪| 办公室深深挺进女警小泬| 国产小受呻吟AV视频在线观看| 精品无人区乱码1区2区3区| 成品网站W灬源码三叶草| 肉乳床欢无码A片A片120秒| 久久久久久亚洲AV无码专区| 人与嘼交av免费| 国产SM主人调教女M视频| 激情A片久久久久久播放| 国产午夜精品一区二区| 丰满老熟好大BBB| 午夜精品A片一区二区三区| 国产精品国产三级国产AV主播| 日产欧产美韩系列| 娇妻被朋友日出白浆H| 中文字幕一区二区三区四区五区| 国产寡妇XXXX猛交|